Endocrine regulation and mechanism of hydrocarbon pheromone
production in the housefly, Musca domestica
G. J. Blomquist 1 , A.
G. Bagneres 2 , C. Schal 3 , M. Kuenzli 1 , X. Shen 1 & C.
1 Dept. Biochem,
Univ. Nevada, Reno, NV 89557, USA; 2 Lab. de Neurobiolgie,
CNRS-UPR, Comm. Chimique, Marseille, Cedex 20, France; 3 Dept.
Entomol., Univ. North Carolina, Raleigh, NC 27695
The major pheromone
component, (Z)-9-tricosene, of the female housefly, Musca
domestica, is produced about two days after emergence to the
adult. Ovarian-produced ecdysteroids induce a change in the chain
length of the cuticular alkenes from producing primarily
(Z)-9-heptacosene to produce (Z)-9-tricosene (muscalure) by
altering the chain length specificity of fatty acyl-CoA elongases.
(Z)-9-Tricosene appears in the hemolymph, associated with
lipophorin, about one day prior to similar amounts appearing on
the surface of the insect. Other pheromone components include
epoxide and ketone derivatives of (Z)-9-tricosene. The C23 epoxide
and ketone pheromone components are not present in the hemolymph,
suggesting that (Z)-9-tricosene is converted to the epoxide and
ketone in the cells that transport hydrocarbon from the hemolymph
to the surface of the insect. Cytochrome P450s are involved in
both converting a C24 aldehyde to (Z)-9-tricosene and subsequently
converting (Z)-9-tricosene to the epoxide and ketone. Three
integument enriched cytochrome P450s have been cloned and
sequenced and are in the process of being expressed and assayed.
Copyright: The copyrights of
this original work belong to the authors (see right-most box in
title table). This abstract appeared in Session 4 – CHEMICAL AND
PHYSIOLOGICAL ECOLOGY Symposium and Poster Session, ABSTRACT
BOOK I – XXI-International Congress of Entomology, Brazil,
August 20-26, 2000.