Thioester-containing proteins in innate immunity of mosquito
1 , L.F. Moita 1 , S. Blandin 1 , M. Lagueux 2 , J.A. Hoffmann 2 &
F.C. Kafatos 1
EMBL, 1 Meyerhofstrasse, 69117 Heidelberg, Germany;
2 UPR9022 du CNRS, IBMC, 15 rue Rene Descartes, 67084 Strasbourg
Here we report a new
family of proteins from human malaria insect vector Anopheles
gambiae that share sequence and structural similarities with the
family of C3/alpha2-macroglobulins. We show that these proteins play
an important role in cellular immune response of mosquito.
Developmental and immune-inducible expression of the genes encoding
Anopheles thioester-containing proteins (aTEPs) will be presented
with particular emphasis on aTEP-I. Western blotting analysis of
aTEP-I demonstrates functionality of the aTEP-I thioester site and
its specific role in binding to Gram-negative bacteria. Results of
the phagocytic assays using a hemocyte-like mosquito cell line and
of a RNAi knockout of aTEP-I will be discussed.
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work belong to the author (see right-most box of the title table).
It also appears in Session 13 - INSECT PHISIOLOGY, NEUROSCIENCES,
IMMUNITY AND CELL BIOLOGY Symposium and Poster Session, ABSTRACT
BOOK II – XXI-International Congress of Entomology, Brazil, August